Spillane graduated from the University of Queensland, Australia, with a degree in veterinary medicine. After a small animal rotating internship in Queensland, she completed an emergency and critical care residency at the University of Illinois. In 2022, she achieved diplomate status with the American College of Veterinary Emergency and Critical Care. Spillane then completed a fellowship at The Ohio State University in extracorporeal therapies, where she developed a deep passion for nephrology. Currently, she is advancing her specialization as one of the inaugural residents of the American College of Veterinary Nephrology and Urology. In October 2023, Spillane joined ACCESS - Los Angeles, a Thrive Pet Healthcare partner, to lead the nephrology and extracorporeal therapies department.